primary antibodies against cd68  (Proteintech)

Journal: bioRxiv

Article Title: Re-evaluating the Impact of Biological Sex on Atherosclerosis in Apoe −/− and Ldlr −/− mice

doi: 10.1101/2025.09.19.677480

Figure Lengend Snippet: A. Eight-week-old male and female Apoe −/− mice were fed an AMLN diet for 20 weeks to induce atherosclerosis. Fat mass, lean mass and random blood glucose were measured at endpoint (n = 9-10/group). Fat mass and lean mass were analyzed by Mann-Whitney test and random blood glucose was analyzed by unpaired t test. B. Glucose tolerance tests at week 13 (n = 9-10/group). Area under curve (AUC) comparison between sex were performed using Mann-Whitney test. Insulin tolerance tests at week 14 (n = 9-10/group). AUC comparison was analyzed by Mann-Whitney test. C. Serum lipid profiles (TC, TG, LDL, HDL) and Liver TC and TG were measured at endpoint (n = 9-10/group). unpaired t test was used in the data analyzation. D. Blood cell composition was measured at endpoint (n = 8-10/group). Gran% and Lym% were analyzed by unpaired t test. Mon% was analyzed by Mann-Whitney test. E. Aortic en face Oil Red O staining and plaque quantification (n = 9-10/group). Scale bar: 1 mm. unpaired t test was used in the data analyzation. F. Aortic sinus sections Oil Red O staining and plaque area quantification (n = 9-10/group). Scale bar: 250 μm. unpaired t test was used in the data analyzation. HE staining of aortic sinus sections and necrotic core area quantification (n = 7-10/group). Main panel scale bar: 500 μm; inset: 62.5 μm. unpaired t test was used in the data analyzation. Masson’s trichrome staining of aortic sinus sections and collagen content quantification (n = 5/group). Scale bar: 500 μm. unpaired t test was used in the data analyzation. CD68 immunofluorescence staining of aortic sinus sections and macrophage infiltration quantification (n = 9-10/group). Scale bar: 250 μm. Mann-Whitney test was used in the data analyzation. All N numbers given represent biological replicates.

Article Snippet: Primary antibodies against CD68 (BIORAD, MCA1957GA) were diluted and applied to the sections, followed by overnight incubation at 4°C.

Techniques: MANN-WHITNEY, Comparison, Staining, Immunofluorescence

Journal: bioRxiv

Article Title: Re-evaluating the Impact of Biological Sex on Atherosclerosis in Apoe −/− and Ldlr −/− mice

doi: 10.1101/2025.09.19.677480

Figure Lengend Snippet: A.Eight-week-old male and female Ldlr −/− mice were fed an AMLN diet for 20 weeks to induce atherosclerosis. Fat mass, lean mass and random blood glucose were measured at endpoint (n = 10/group). Fat mass and random blood glucose were analyzed by unpaired t test and lean mass was analyzed by Mann-Whitney test. B. Glucose tolerance tests at week 13 (n = 10/group). Area under curve (AUC) comparison between sex were performed using unpaired t test. Insulin tolerance tests at week 14 (n = 9-10/group). AUC comparison was analyzed by Welch’s t test. C. Serum lipid profiles (TC, TG, LDL, HDL) and Liver TC and TG were measured at endpoint (n = 10/group). TC was analyzed by Welch’s t test. TG was analyzed by Mann-Whitney test. LDL, HDL, Liver TC and Liver TG were analyzed by unpaired t test. D. Blood cell composition was measured at endpoint (n = 10/group). Gran% and Lym% were analyzed by Welch’s t test. Mon% was analyzed by Mann-Whitney test. E. Aortic en face Oil Red O staining and plaque quantification (n = 10/group). Scale bar: 1 mm. unpaired t test was used in the data analyzation. F. Aortic sinus sections Oil Red O staining and plaque area quantification (n = 9-10/group). Scale bar: 250 μm. unpaired t test was used in the data analyzation. HE staining of aortic sinus sections and necrotic core area quantification (n = 10/group). Main panel scale bar: 500 μm; inset: 62.5 μm. unpaired t test was used in the data analyzation. Masson’s trichrome staining of aortic sinus sections and collagen content quantification (n = 4-5/group). Scale bar: 500 μm. unpaired t test was used in the data analyzation. CD68 immunofluorescence staining of aortic sinus sections and macrophage infiltration quantification (n = 10/group). Scale bar: 250 μm. unpaired t test was used in the data analyzation. All N numbers given represent biological replicates.

Article Snippet: Primary antibodies against CD68 (BIORAD, MCA1957GA) were diluted and applied to the sections, followed by overnight incubation at 4°C.

Techniques: MANN-WHITNEY, Comparison, Staining, Immunofluorescence

Journal: Experimental & Molecular Medicine

Article Title: Discovery of ETI41 and ETI60: novel selective endosomal Toll-like receptor inhibitors for the treatment of autoimmune diseases

doi: 10.1038/s12276-025-01526-w

Figure Lengend Snippet: a Psoriasis was induced in female C57BL/6J mice by topically applying IMQ (62.5 mg/cm 2 ). ETI41 and ETI60 were orally administered daily at a dose of 60 mg/kg. b Impact of ETI41 and ETI60 on body weight during treatment. c PASI score indicating disease severity. d Images of the back skin of mice were captured on the fifth day of treatment, revealing the therapeutic efficacy of ETI41 or ETI60 compared with the normal and untreated groups. Immunohistochemical staining of back skin sections from each group revealed the effects of ETI41 and ETI60 on thickness of the epidermis (yellow arrowhead) and dermis (green arrowhead). e The thickness of the epidermis and dermis, as well as the expression levels of CD68 (a macrophage marker), Ki-67, IL-17A and IL-23, were measured. f Psoriasis was induced in female C57BL/6J mice by injecting IL-23 (500 ng) into their ear skin. Anti-IL17A antibody (Ab, 60 µg) was administered by IP injection as a positive control on days 2, 5 and 8. ETI41 or ETI60 was administered orally at a daily dose of 60 mg/kg for 9 days. g Body weight was recorded during treatment. h On the ninth day of treatment, images of the mouse ears were acquired to demonstrate the therapeutic efficacy of ETI41 and ETI60 in comparison with the normal, vehicle or anti-IL17 groups. CD68 and Ki-67 expression patterns were determined in skin sections from a representative of each group using immunofluorescence. i Ear skin and epidermis thickness, CD68 expression and percentage of Ki-67 cells were examined from 4’,6-diamidino-2-phenylindole-stained cells. Statistical differences between the induced case and other cases were analyzed and verified using a one-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001).

Article Snippet: Immunohistochemical analyses were performed using primary antibodies against CD68 (R&D Systems, MAB101141), Ki-67 (Cell Signaling Technology, 12202), IL-17A (Novus Biologicals, NBP1-72027) and IL-23 (Novus Biologicals, NBP1-76697), followed by detection using biotinylated secondary antibodies (Vector Laboratories) and diaminobenzidine (Vector Laboratories) visualization.

Techniques: Drug discovery, Immunohistochemical staining, Staining, Expressing, Marker, Injection, Positive Control, Comparison, Immunofluorescence, One-tailed Test