Journal: bioRxiv
Article Title: Re-evaluating the Impact of Biological Sex on Atherosclerosis in Apoe −/− and Ldlr −/− mice
doi: 10.1101/2025.09.19.677480
Figure Lengend Snippet: A.Eight-week-old male and female Ldlr −/− mice were fed an AMLN diet for 20 weeks to induce atherosclerosis. Fat mass, lean mass and random blood glucose were measured at endpoint (n = 10/group). Fat mass and random blood glucose were analyzed by unpaired t test and lean mass was analyzed by Mann-Whitney test. B. Glucose tolerance tests at week 13 (n = 10/group). Area under curve (AUC) comparison between sex were performed using unpaired t test. Insulin tolerance tests at week 14 (n = 9-10/group). AUC comparison was analyzed by Welch’s t test. C. Serum lipid profiles (TC, TG, LDL, HDL) and Liver TC and TG were measured at endpoint (n = 10/group). TC was analyzed by Welch’s t test. TG was analyzed by Mann-Whitney test. LDL, HDL, Liver TC and Liver TG were analyzed by unpaired t test. D. Blood cell composition was measured at endpoint (n = 10/group). Gran% and Lym% were analyzed by Welch’s t test. Mon% was analyzed by Mann-Whitney test. E. Aortic en face Oil Red O staining and plaque quantification (n = 10/group). Scale bar: 1 mm. unpaired t test was used in the data analyzation. F. Aortic sinus sections Oil Red O staining and plaque area quantification (n = 9-10/group). Scale bar: 250 μm. unpaired t test was used in the data analyzation. HE staining of aortic sinus sections and necrotic core area quantification (n = 10/group). Main panel scale bar: 500 μm; inset: 62.5 μm. unpaired t test was used in the data analyzation. Masson’s trichrome staining of aortic sinus sections and collagen content quantification (n = 4-5/group). Scale bar: 500 μm. unpaired t test was used in the data analyzation. CD68 immunofluorescence staining of aortic sinus sections and macrophage infiltration quantification (n = 10/group). Scale bar: 250 μm. unpaired t test was used in the data analyzation. All N numbers given represent biological replicates.
Article Snippet: Primary antibodies against CD68 (BIORAD, MCA1957GA) were diluted and applied to the sections, followed by overnight incubation at 4°C.
Techniques: MANN-WHITNEY, Comparison, Staining, Immunofluorescence